The aim of this work is to formulate a collagen based dermal scaffold containing Glycyrrhiza glabra (Licorice) root extract for the improvement in the quality of wound healing. For this Collagen was isolated from bovine Achilles tendon and tested for its confirmation of presence, purity and sterility. The physicochemical compatibility between collagen and Glycyrrhiza glabra root extract was studied by FT-IR and results obtained suggested no interaction. Collagen based Scaffolds were formulated using different concentrations of Glycyrrhiza glabra root extract 5%w/v, 10%w/v and 15%w/v (GGEICDS- Glycyrrhiza glabra extract incorporated collagen dermal scaffolds). The prepared scaffolds were subjected to physical, biochemical and histopathological examinations. Microshrinkage Temperature of the scaffolds containing 5%w/v,10%w/v & 15%w/v of GGEICDS were found to be 690C, 730C & 780C respectively indicated that they were hydrothermally stable. Wound healing studies on Male Wister Rats were performed for a period of 7 days and it was observed that the 10%w/v GGEICDS treated rats possessed higher amount of hydroxyl proline content 68.9%, when compared to that of the existing Marketed formulation (Neu- skinTM) (59.7%) . Further better wound healing activity was observed in the 10%w/v GGEICDS treated rats (75%) with that of the others including the Marketed formulation (55.2%). When examined histopathologically, more production in the collagen content was clearly observed in 10% w/v GGEICDS treated groups which resulted in the epithelial gap reduction. Further the rise in fibroblast count (62 in 100µ in the wound during the healing process) in 10% GGEICDS treated groups was observed. This research suggested that 10% GGEICDS is a feasible and productive approach in the improvement of dermal wound healing process.
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